티타늄 Particle농도에 따른 치주인대세포내 세포독성 및 OPG, RANKL의 발현에 관한 연구
A Study of the Expression of OPG and RANKL due to Titanium Particles in Periodontal Ligament Cells
조형준, 나유진, 정용석, 홍지숙, 김윤지, 이종헌, 천재식, 박양호,
소속 상세정보
조형준 ( Cho Hyung-Jun ) - 닥터스마일치과
나유진 ( Na Yu-Jin ) - 경희대학교 자연과학대학 생물학과
정용석 ( Jeong Yong-Suk ) - 경희대학교 자연과학대학 생물학과
홍지숙 ( Hong Ji-Suk ) - 한림대학교의료원 강동성심병원 치과교정과
김윤지 ( Kim Yoon-Ji ) - 한림대학교의료원 강동성심병원 치과교정과
이종헌 ( Lee Chong-Heon ) - 단국대학교 치과대학 구강병리학교실
천재식 ( Cheon Gae-Sik ) - 단국대학교 치과대학 구강생리학교실
박양호 ( Park Yang-Ho ) - 한림대학교의료원 강동성심병원 치과교정과
KMID : 0829220110350020067
Abstract
The purpose of this study was to investigate the effect of cytokine-induced osteoclastogenesis on tooth movement related to orthodontic force. We evaluated the cytotoxicity as well as the expression of OPG and RANKL, which influence the homeostasis of bone metabolism. Titanium particles were applied to human periodontal ligament cells and subcultured fourth generation cells. The ALP assay and the MTT assay were used to assess changes in cytotoxicity. After 48 hours, cytotoxicity increased proportionally with the concentration of titanium. With 20 ㎎, the cytotoxicity was the lowest. RT-PCR was used for assessing mRNA levels of OPG and RANKL; after 96 hours, the mRNAs of OPG and RANKL increased steeply. A western blot analysis showed that with 20 ㎎ of titanium, the protein expression of OPG increased linearly with time, especially after 96 hours, while the protein expression of RANKL did not show significant changes with titanium processing. Given the increase in OPG expression after the initial cytotoxicity, changes in cytotoxicity with titanium may be attributable to the antagonistic effect of OPG on cytotoxicity.
키워드
OPG; Peridontal ligament cells; RANKL; Titanium particles
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